CAS No.: 7220-79-3
Product introduction:Methylene Blue trihydrate is a well-known synthetic thiazine dye with a long history of medical and research applications. It is the trihydrated crystalline form of methylene blue (C₁₆H₁₈ClN₃S · 3H₂O), which offers enhanced stability and handling properties compared to the anhydrous form. Beyond its traditional use as a biological stain, methylene blue has gained significant attention in pharmacology and neuroscience due to its unique redox properties and multiple biological activities. The compound has been shown to function as a monoamine oxidase inhibitor (MAOI), a nitric oxide synthase inhibitor, and an electron cycler in the mitochondrial electron transport chain, where it can enhance cellular respiration under hypoxic conditions. In recent years, methylene blue has been repurposed for various clinical and research applications, including the treatment of methemoglobinemia, as a photosensitizer in photodynamic therapy, and as a potential neuroprotective agent for cognitive enhancement.
CAS No.:7220-79-3
Methylene Blue trihydrate appears as dark green to bronze-colored crystals with a characteristic crystalline structure. Its molecular formula is C₁₆H₁₈ClN₃S · 3H₂O with a molecular weight of approximately 373.90 g/mol. The compound is freely soluble in water and chloroform, sparingly soluble in ethanol, and practically insoluble in most other organic solvents. Aqueous solutions of methylene blue exhibit a characteristic deep blue color with a maximum absorbance at approximately 665 nm. The trihydrate form is more stable than the anhydrous form and should be stored in a tightly sealed container, protected from light, in a cool and dry environment. Methylene blue trihydrate is typically available in various purity grades, including USP, EP, and research-grade (≥98% by HPLC).
Core Functions:
Pharmaceutical ingredient for the treatment of drug-induced methemoglobinemia and as an antidote for cyanide poisoning (in combination with sodium thiosulfate)
Biological stain and histological dye for microscopy, including the staining of nucleic acids, bacteria, and nervous tissue specimens
Photosensitizer in photodynamic therapy for the treatment of certain cancers and bacterial infections
Research compound in neuroscience for studies of memory consolidation, cognitive enhancement, and neuroprotection via monoamine oxidase inhibition
Diagnostic agent for the detection of urologic and gastrointestinal fistulas
Active ingredient in aquaculture for the prevention and treatment of fungal infections in fish eggs and fry
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